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relative quantification with delta Cq and delta delta Cq

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@ewallace ewallace released this 04 Sep 13:05
v0.2-beta
cafce12
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Learn about vigilant mode.

Upgraded features for relative quantification, new vignettes to explain these, and gave functions more helpful names such as calculate... as well as create...

  • lay out and display 96/384-well plates for easy experimental setup (label_plate_rowcol, create_blank_plate, ...)
  • read-in Cq and raw data from Roche LightCycler machines with single-channel fluorescence (read_lightcycler_1colour_cq, read_lightcycler_1colour_raw)
  • delta Cq: normalization/ relative quantification of Cq data to one or more reference targets by delta count method (calculate_normcq, calculate_deltacq_bysampleid)
  • delta delta Cq: normalization of delta Cq data across multiple samples (calculate_deltadeltacq_bytargetid)
  • calibration of primer sets including estimating efficiencies and visualization of curves (calculate_efficiency, and see vignettes)
  • visualization of amplification and melt curves (calculate_drdt_plate, and see vignettes)
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