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R-Bioinformatics

Semester1 Reviewing

TCGA database

TCGA data Pre-processing for differential analysis

TCGA Differential analysis

COX regression analysis

ESTIMATE immune score

ROC curve drawing

Time-dependent ROC

TCGA differential analysis heat map

TCGA differential analysis volcano map

CIBERSORT immune cell ratio

Correlation heat map

Survival analysis

Gene high and low group differential analysis (preliminary preparation for enrichment analysis)

Unsupervised clustering

Grouping cibersort

ssGSEA

KEGG

GSEA

LASSO

TMB tumor mutation burden

LASSO dot plot display

Nomogram

GEO Dataset

Download and organize GEO database data

GEO differential analysis and heat map display

GEO three major enrichment analysis

GEO data set merging

Immune checkpoint expression differences Genecards usage

Manual annotation of GEO gene names

GEPIA Dataset

GEPIA database-01 co-expressed genes

GEPIA database-02 survival analysis

GEPIA database-03 differential analysis

Others

fpkm to tpm

STRING to build molecular network

Cytoscape to build molecular network

Xcell WGCNA

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