Question about the ambient RNA correction

[Chaosli98]

Hi,
First of all, thank you very much for the excellent tutorial. It provides great guidance for professionals in the related field. I have some questions regarding ambient RNA correction:
1.At which point should I perform ambient RNA correction? I noticed that in the current tutorial, low-quality cells and some outliers are removed first before performing ambient RNA correction. Shouldn't ambient RNA correction be done first, followed by cell removal based on pct_counts_mt? Or do you believe the current approach is better?
2.I noticed that ambient RNA correction has not yet been included in the Scanpy tutorial. Is this step essential for droplet-based technologies like 10X?
3.Lastly, I would like to ask about sc.pp.regress_out and sc.pp.scale, which also don't seem to be considered necessary steps in the tutorial. Are these steps recommended? I really look forward to your response, as these questions are crucial for many people who are struggling with different pipelines.