Anesthetics uniquely decorrelate hippocampal network activity, alter spine dynamics and affect memory consolidation
This repository contains code related to the paper "Anesthetics fragment hippocampal network activity, alter spine dynamics and affect memory consolidation". The manuscript is available here: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001146
Click on the image below to open it in interactive mode:
Code to align calcium imaging recordings from different sessions but with the same FOV is in the Alignment folder.
| Before alignment | After alignment |
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Python code for analysis of calcium transients and correlation matrices is in Figures 3-5 folder
Matlab code for clustering in the spatial and temporal domain and sleep classification is in the Figures 5-7 folder
Further Matlab code that was used for the ephys-part of the paper can be found in this other repository
R scripts and datasets that were used for all statistical analysis are available in the Stats folder.
Raw 2-photon and electrophysiology data is available at this repository on GIN.
Analysis of neuronal activity on single neuron and population level
Extraction of the main features permits the analysis of the recordings on both population and single neuron levels.
(notebooks/analysis_pipeline_for_concatenated_recordings.ipynb)
Validation of calcium recordings: homogeneity and stability
This notebook illustrates the extraction of the main features of the calcium traces for a large set of recordings and subsequent analysis of its variations and stability.
(notebooks/Validation_stability.ipynb)