Usage issue, repeatedly encountering 'Error: Error detecting input file format. First line seems to be blank.'

[BenWu1224]

Hello everyone, I apologize for not having much experience in this aspect of analysis.

Back in September, I attempted to annotate my total RNA seq data. At that time, I successfully ran the complete emapper.py script with one of my files and obtained the subsequent results (.emapper.annotations).

However, after confirming that the method was viable, I tried to annotate all my samples but kept encountering the following error message. Despite multiple attempts, I couldn't identify the problem. Even using the input that successfully ran in September led to the same issue. Has anyone encountered a similar problem and knows how to resolve it?

Thank you for your assistance, and I apologize for the trouble.

emapper.py -m diamond --itype CDS --translate -i NGmerge_Results/Day049_D1_merged_1001.fq -o Eggnog_results/ --cpu 18

emapper-2.1.12

emapper.py -m diamond --itype CDS --translate -i NGmerge_Results/Day049_D1_merged_1001.fq -o Eggnog_results/ --cpu 18

/home/hpllab/miniconda3/envs/eggnog_env/bin/diamond blastp -d '/home/hpllab/eggnog-mapper-data/eggnog_proteins.dmnd' -q '/mnt/d/rna/202407-NewTry/NGmerge/emappertmp_dmdn_laah8soi/tmplap9kwrs' --threads 18 -o '/mnt/d/rna/202407-NewTry/NGmerge/Eggnog_results/.emapper.hits' --tmpdir '/mnt/d/rna/202407-NewTry/NGmerge/emappertmp_dmdn_laah8soi' --sensitive --iterate -e 0.001 --top 3 --outfmt 6 qseqid sseqid pident length mismatch gapopen qstart qend sstart send evalue bitscore qcovhsp scovhsp
Error running diamond: Opening the input file... Error: Error detecting input file format. First line seems to be blank.