diff --git a/test/e2e/nodelatencymonitor_test.go b/test/e2e/nodelatencymonitor_test.go
new file mode 100644
index 00000000000..c9ea90accc9
--- /dev/null
+++ b/test/e2e/nodelatencymonitor_test.go
@@ -0,0 +1,131 @@
+// Copyright 2024 Antrea Authors
+//
+// Licensed under the Apache License, Version 2.0 (the "License");
+// you may not use this file except in compliance with the License.
+// You may obtain a copy of the License at
+//
+//	http://www.apache.org/licenses/LICENSE-2.0
+//
+// Unless required by applicable law or agreed to in writing, software
+// distributed under the License is distributed on an "AS IS" BASIS,
+// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+// See the License for the specific language governing permissions and
+// limitations under the License.
+package e2e
+
+import (
+	"context"
+	"testing"
+	"time"
+
+	"github.com/stretchr/testify/require"
+
+	metav1 "k8s.io/apimachinery/pkg/apis/meta/v1"
+	"k8s.io/apimachinery/pkg/util/wait"
+
+	crdv1alpha1 "antrea.io/antrea/pkg/apis/crd/v1alpha1"
+	"antrea.io/antrea/pkg/apis/stats/v1alpha1"
+	"antrea.io/antrea/pkg/features"
+)
+
+func TestNodeLatencyMonitor(t *testing.T) {
+	skipIfFeatureDisabled(t, features.NodeLatencyMonitor, true, false)
+	skipIfHasWindowsNodes(t) // as the feature does not have official Windows support
+
+	data, err := setupTest(t)
+	require.NoError(t, err, "Error when setting up test")
+
+	defer teardownTest(t, data)
+
+	var isDualStack bool
+	if clusterInfo.podV4NetworkCIDR != "" && clusterInfo.podV6NetworkCIDR != "" {
+		isDualStack = true
+	}
+
+	expectedTargetIPLatencyStats := 1
+	if isDualStack {
+		expectedTargetIPLatencyStats = 2
+	}
+
+	_, err = data.crdClient.CrdV1alpha1().NodeLatencyMonitors().Create(context.TODO(), &crdv1alpha1.NodeLatencyMonitor{
+		ObjectMeta: metav1.ObjectMeta{
+			Name: "default",
+		},
+		Spec: crdv1alpha1.NodeLatencyMonitorSpec{
+			PingIntervalSeconds: int32(10),
+		},
+	}, metav1.CreateOptions{})
+	require.NoError(t, err, "Failed to create NodeLatencyMonitor CR")
+	t.Logf("NodeLatencyMonitor CR created successfully.")
+
+	defer func() {
+		err = data.crdClient.CrdV1alpha1().NodeLatencyMonitors().Delete(context.TODO(), "default", metav1.DeleteOptions{})
+		require.NoError(t, err, "Failed to delete NodeLatencyMonitor CR")
+		t.Logf("NodeLatencyMonitor CR deleted successfully.")
+	}()
+	previousTimes := make(map[string]map[string]metav1.Time)
+
+	validateNodeLatencyStats := func(statsList *v1alpha1.NodeLatencyStatsList, initialPoll bool) (bool, error) {
+		if len(statsList.Items) != clusterInfo.numNodes {
+			t.Logf("Expected %d NodeLatencyStats, but found %d, retrying...", clusterInfo.numNodes, len(statsList.Items))
+			return false, nil
+		}
+
+		for _, item := range statsList.Items {
+			if len(item.PeerNodeLatencyStats) != clusterInfo.numNodes-1 {
+				t.Logf("Expected %d PeerNodeLatencyStats for Node %s, but found %d, retrying...", clusterInfo.numNodes-1, item.Name, len(item.PeerNodeLatencyStats))
+				return false, nil
+			}
+
+			for _, peerStat := range item.PeerNodeLatencyStats {
+				if len(peerStat.TargetIPLatencyStats) != expectedTargetIPLatencyStats {
+					t.Logf("Expected %d TargetIPLatencyStats for peer %s on Node %s, but found %d, retrying...",
+						expectedTargetIPLatencyStats, peerStat.NodeName, item.Name, len(peerStat.TargetIPLatencyStats))
+					return false, nil
+				}
+
+				for _, targetStat := range peerStat.TargetIPLatencyStats {
+					if targetStat.LastMeasuredRTTNanoseconds <= 0 {
+						t.Logf("Invalid RTT for peer %s reported by Node %s", peerStat.NodeName, item.Name)
+						return false, nil
+					}
+
+					if initialPoll {
+						if previousTimes[item.Name] == nil {
+							previousTimes[item.Name] = make(map[string]metav1.Time)
+						}
+						previousTimes[item.Name][peerStat.NodeName] = targetStat.LastRecvTime
+					} else {
+						previousRecvTime, recvTimeExists := previousTimes[item.Name][peerStat.NodeName]
+						if !recvTimeExists || !targetStat.LastRecvTime.After(previousRecvTime.Time) {
+							t.Logf("LastRecvTime has not been updated for peer %s on Node %s", peerStat.NodeName, item.Name)
+							return false, nil
+						}
+					}
+				}
+			}
+		}
+		return true, nil
+	}
+
+	err = wait.PollUntilContextTimeout(context.TODO(), time.Second, 30*time.Second, false, func(ctx context.Context) (bool, error) {
+		statsList, err := data.crdClient.StatsV1alpha1().NodeLatencyStats().List(ctx, metav1.ListOptions{})
+		if err != nil {
+			return false, err
+		}
+		return validateNodeLatencyStats(statsList, true)
+	})
+	require.NoError(t, err, "Failed to validate initial NodeLatencyStats")
+
+	err = wait.PollUntilContextTimeout(context.TODO(), time.Second, 30*time.Second, false, func(ctx context.Context) (bool, error) {
+		statsList, err := data.crdClient.StatsV1alpha1().NodeLatencyStats().List(ctx, metav1.ListOptions{})
+		if err != nil {
+			return false, err
+		}
+		return validateNodeLatencyStats(statsList, false)
+	})
+	require.NoError(t, err, "Failed to validate updated NodeLatencyStats")
+
+	t.Logf("Successfully received and validated NodeLatencyStats")
+
+}